Part:BBa_K847211:Design
nrd operon promoter
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Potential cell-cycle dependent promoters in Escherichia coli and their sequences were identified from literature (Quiñones 1997) and isolated using Colony PCR. We developed our constructs for assay by ligating these PCR products in front of BBa_E0840, which consists of RBS BBa_B0030, reporter BBa_E0040 (mut3b GFP), and terminator BBa_B0015.
This construct performs effectively in E. coli; functionality in other organisms has not been tested.
Source
E. coli strain K12
References
Messer M., Weigel C.: DnaA initiator - also a transcription factor. Molecular Microbiology 1997, 24:1-6.
Chiaramello A.E., Zyskind, J.W.: Coupling of DNA replication to growth rate in Escherichia coli: A possible role for guanosine tetraphosphate. J. Bacteriology 1990, 172:2013-2019.
Sun L., Jacobson B., Dien B., Srienc F., Fuchs J.: Cell Cycle Regulation of the Escherichia coli nrd Operon: Requirement for a cis-Acting Upstream AT-Rich Sequence. J. Bacteriology 1994, 176: 2415-2426.
Sun L., Fuchs J.: Escherichia coli Ribonucleotide Reductase Expression is Cell Cycle Regulated. Molecular Biology of the Cell 1992, 3:1095-1105.
Messer W.: The bacterial replication initiator DnaA. DnaA and oriC, the bacterial mode to initiate DNA replication 2002, 26:355-374.
Ferullo D.J., Cooper D.L., Moore H.R., Lovett S.T.: Cell cycle synchronization of E. coli using the stringent response, with fluorescence labeling assays for DNA content and replication. Methods 2009, 48:8-13.
Grant M.A.A., Saggioro C., Ferrari U., Bassetti B., Sclavi B., Lagomarsino M.C.: DnaA and the timing of chromosome replication in Escherichia coli as a function of growth rate. BMC Systems Biology 2011, 5:201.